Recent Publications:
2007

  • Fu N, Drinnenberg I, Kelso J, Wu JR, Paabo S, Zeng R, Khaitovich P. Comparison of protein and mRNA expression evolution in humans and chimpanzees. PLoS ONE. 2007 Feb 14;2:e216.[pdf]
  • Dai J, Jin WH, Sheng QH, Shieh CH, Wu JR, Zeng R.. Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry. J Proteome Res. 2007 Jan;6(1):250-62.[pdf]
  • 2006

  • Sheng QH, Wang LS, Dai J, Jiang XS, Li RX, Ma DJ, Li YX, Zeng R, Wu JR. Comparison of a proteomic approach with a microarray-based approach to detect exons in the mouse genome. Nat Genet. 2006 Nov;38(11):1223-4. [pdf]
  • 2005

  • Dai J, Shieh CH, Sheng QH, Zhou H, Zeng R. Proteomic analysis with integrated multiple dimensional liquid chromatography/mass spectrometry based on elution of ion exchange column using pH steps. Anal Chem. 2005, 77:5793-9. [pdf]
  • Tu CJ, Dai J, Li SJ, Sheng QH, Deng WJ, Xia QC, Zeng R. High-sensitivity analysis of human plasma proteome by immobilized isoelectric focusing fractionation coupled to mass spectrometry identification. J Proteome Res. 2005, 4:1265-73.[pdf]
  • Li RX, Zhou H, Li SJ, Sheng QH, Xia QC, Zeng R. Prefractionation of proteome by liquid isoelectric focusing prior to two-dimensional liquid chromatography mass spectrometric identification. J Proteome Res. 2005, 4:1256-64.[pdf]
  • Zong X, Eckert C, Yuan H, Wahl-Schott C, Abicht H, Fang L, Li R, Mistrik P, Gerstner A, Much B, Baumann L, Michalakis S, Zeng R, Chen Z, Biel M. A novel mechanism of modulation of hyperpolarization-activated cyclic nucleotide-gated channels by SRC kinase. J Biol Chem. 2005, 280: 34224-32[pdf]
  • He P, He HZ, Dai J, Wang Y, Sheng QH, Zhou LP, Zhang ZS, Sun YL, Liu F, Wang K, Zhang JS, Wang HX, Song ZM, Zhang HR, Zeng R, Zhao X. The human plasma proteome: Analysis of Chinese serum using shotgun strategy. Proteomics. 2005, 5: 3442-53[pdf]
  • Chen YB, Yang CP, Li RX, Zeng R, Zhou JQ. Def1p is involved in telomere maintenance in budding yeast. J Biol Chem. 2005, 280:24784-91.[pdf]
  • Jin WH, Dai J, Li SJ, Xia QC, Zou HF, Zeng R. Human plasma proteome analysis by multidimensional chromatography prefractionation and linear ion trap mass spectrometry identification. J Proteome Res. 2005, 4:613-9.[pdf]
  • Li D, Fu Y, Sun R, Ling CX, Wei Y, Zhou H, Zeng R, Yang Q, He S, Gao W. pFind: a novel database-searching software system for automated peptide and protein identification via tandem mass spectrometry. Bioinformatics. 2005, 21:3049-50.[pdf]
  • Li Z, Dong X, Wang Z, Liu W, Deng N, Ding Y, Tang L, Hla T, Zeng R, Li L, Wu D. Regulation of PTEN by Rho small GTPases. Nat Cell Biol. 2005, 7:399-404. Erratum in: Nat Cell Biol. 2005, 7:531.
  • Jiang XS, Tang LY, Dai J, Zhou H, Li SJ, Xia QC, Wu JR, Zeng R. Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection. Mol Cell Proteomics. 2005, 4:902-13.[pdf]
  • Li C, Tan YX, Zhou H, Ding SJ, Li SJ, Ma DJ, Man XB, Hong Y, Zhang L, Li L, Xia QC, Wu JR, Wang HY, Zeng R. Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers. Proteomics. 2005, 5:1125-39.[pdf]
  • Jiang XS, Dai J, Sheng QH, Zhang L, Xia QC, Wu JR, Zeng R. A comparative proteomic strategy for subcellular proteome research: ICAT approach coupled with bioinformatics prediction to ascertain rat liver mitochondrial proteins and indication of mitochondrial localization for catalase. Mol Cell Proteomics. 2005, 4:12-34.[pdf]
  • 2004

  • Jin WH, Dai J, Zhou H, Xia QC, Zou HF, Zeng R. Phosphoproteome analysis of mouse liver using immobilized metal affinity purification and linear ion trap mass spectrometry. Rapid Commun Mass Spectrom. 2004, 18:2169-76.[pdf]
  • Xu NJ, Yu YX, Zhu JM, Liu H, Shen L, Zeng R, Zhang X, Pei G. Inhibition of SNAP-25 phosphorylation at Ser187 is involved in chronic morphine-induced down-regulation of SNARE complex formation. J Biol Chem. 2004, 279:40601-8[pdf]
  • Zeng R, Yang RF, Shi MD, Jiang MR, Xie YH, Ruan HQ, Jiang XS, Shi L, Zhou H, Zhang L, Wu XD, Lin Y, Ji YY, Xiong L, Jin Y, Dai EH, Wang XY, Si BY, Wang J, Wang HX, Wang CE, Gan YH, Li YC, Cao JT, Zuo JP, Shan SF, Xie E, Chen SH, Jiang ZQ, Zhang X, Wang Y, Pei G, Sun B, Wu JR. Characterization of the 3a protein of SARS-associated coronavirus in infected vero E6 cells and SARS patients. J Mol Biol. 2004, 341:271-9.[pdf]
  • Zeng R, Ruan HQ, Jiang XS, Zhou H, Shi L, Zhang L, Sheng QH, Tu Q, Xia QC, Wu JR, Proteomic Analysis of SARS Associated Coronavirus Using Two-Dimensional Liquid Chromatography Mass Spectrometry and One-Dimensional Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis Followed by Mass Spectrometric Analysis. Journal of Proteome Research. 2004, 3:549- 55.[pdf]
  • Ding SJ, Li Y, Shao XX, Zhou H, Zeng R, Tang ZY, Xia QC, Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentials. Proteomics. 2004, 4:982-94.[pdf]
  • Fu Y, Yang Q, Sun R, Li D, Zeng R, Ling CX, Gao W, Exploiting the kernel trick to correlate fragment ions for peptide identification via tandem mass spectrometry. Bioinformatics. 2004, 20;1948-54.
  • Hermjakob H, Montecchi-Palazzi L, Bader G, Wojcik J, Salwinski L, Ceol A, Moore S, Orchard S, Sarkans U, von Mering C, Roechert B, Poux S, Jung E, Mersch H, Kersey P, Lappe M, Li Y, Zeng R, Rana D, Nikolski M, Husi H, Brun C, Shanker K, Grant SG, Sander C, Bork P, Zhu W, Pandey A, Brazma A, Jacq B, Vidal M, Sherman D, Legrain P, Cesareni G, Xenarios I, Eisenberg D, Steipe B, Hogue C, Apweiler R, The HUPO PSI's molecular interaction format--a community standard for the representation of protein interaction data. Nat Biotechnol. 2004, 22:177-83.[pdf]
  • Jiang XS, Zhou H, Zhang L, Sheng QH, Li SJ, Li L, Hao P, Li YX, Xia QC, Wu JR, Zeng R, A High-throughput approach for subcellular proteome: Identification of rat liver proteins using subcellular fractionation coupled with two-dimensional liquid chromatography tandem mass spectrometry and bioinformatic analysis. Mol Cell Proteomics. 2004, 3:441-5.[pdf]
  • Li C, Hong Y, Tan YX, Zhou H, Ai JH, Li SJ, Zhang L, Xia QC, Wu JR, Wang HY, Zeng R, Accurate qualitative and quantitative proteomic analysis of clinical hepatocellular carcinoma using laser capture microdissection coupled with isotope-coded affinity tag and two-dimensional liquid chromatography mass spectrometry. Mol Cell Proteomics. 2004, 3:399-409.[pdf]
  • Ding SJ, Li Y, Tan YX, Jiang MR, Tian B, Liu YK, Shao XX, Ye SL, Wu JR, Zeng R, Wang HY, Tang ZY, Xia QC, From Proteomic Analysis to Clinical Significance: Overexpression of Cytokeratin 19 Correlates with Hepatocellular Carcinoma Metastasis. Mol Cell Proteomics. 2004, 3:73-81.[pdf]
  • 2003

  • Li F, Yang XX, Xia HC, Zeng R, Hu WG, Li Z, Zhang ZC. Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica. Peptides. 2003, 24:799-805.
  • Ren SX, Fu G, Jiang XG, Zeng R, Miao YG, Xu H, Zhang YX, Xiong H, Lu G, Lu LF, Jiang HQ, Jia J, Tu YF, Jiang JX, Gu WY, Zhang YQ, Cai Z, Sheng HH, Yin HF, Zhang Y, Zhu GF, Wan M, Huang HL, Qian Z, Wang SY, Ma W, Yao ZJ, Shen Y, Qiang BQ, Xia QC, Guo XK, Danchin A, Saint Girons I, Somerville RL, Wen YM, Shi MH, Chen Z, Xu JG, Zhao GP. Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing. Nature. 2003, 422:888-93.[pdf]
  • Rong Zeng, Yong-Bing Chen, Xiao-Xia Shao, Chia-Hui Paul Shieh, Ken Miller, Helen Tran and Qi-Chang Xia, Identification of proteins separated by one-dimensional sodium dodecyl sulfate/polyacrylamide gel electrophoresis with matrix-assisted laser desorption/ionization ion trap mass spectrometry; comparison with matrix-assisted laser desorption/ionization time-of-flight mass fingerprinting. Rapid Commun. Mass Spectrom. 2003, 17: 1995C2004.[pdf]
  • 2002

  • Tao Liu, Xiao-Xia Shao, Rong Zeng, Qi-Hang Xia, Protein isoforms observed by ultrahighresolution capillary isoelectric focusing electrospray ionization mass spectrometry. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002,34:423-32.
  • Xi Huang, Rong Zeng, Xiao-Ming Ding, Xiang Mao, Yi Ding, Zi-He Rao, Yong Xie, Wei-Hong Jiang, Guo-Ping Zhao. Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl aminocephalosporanic acid acylase of Pseudomonas sp. 130. J Biol Chem, 2002, 277:10256-64 [pdf]
  • Jin-Fang Song, Mei-Qian Weng, Shen-Mei Wu, Qi-Chang Xia. Analysis of neutral saccharides in human milk derivatized with 2-aminoacridone by capillary electrophoresis with laser-induced fluorescence detection. Anal Biochem, 2002, 304:126-9 [pdf]
  • 2001

  • Tao Liu, Jia-Da Li, Rong Zeng, Xiao-Xia Shao, Ke-Yi Wang, Qi-Chang Xia, Capillary electrophoresis and electrospray mass spectrometry for the characterization of high-mannose type N- glycosylation and differential oxaidation in glycoproteins by charge reversal and protease/glycosidase digestion. Anal. Chem, 2001, 24: 5875-85 [pdf]
  • Li-Rong Yu, Xiao-Xia Shao, WL Jiang, Dan.Xu, YC Chang, Yong-Hua Xu, Qi-Chang Xia. Proteome alterations in human hepatoma cells transfected with antisense epidermal growth factor receptor sequence. Electrophoresis, 2001, 22:3001-8 [pdf]
  • Fa-Yun Che, Jin-Fang.Song, Li-Rong Yu, Ke-Yi Wang, Qi-Chang Xia. Flurescent staining glycoproteins on PVDF Membrane with ANTS. BioTechniques, 2001, 30:1272-8
  • Rong Zeng, Qiang Xu., Xiao-Xia Shao, Ke-Yi Wang, Qi-Chang Xia, Determination of the disulfide bond pattern of a novel C-type lectin from snake venom by mass spectrometry.Rapid Comunications in Mass Spectrometry, 2001, 15:2213-20 [pdf]
  • 2000

  • Li-Rong Yu, Rong Zeng,Xiao-Xia Shao, Nan Wang, Yong-Hua Xu, Qi-Chang Xia. Identification of differentially expressed proteins between human hepatoma and normal liver cell lines by two-dimensional electrophoresis and liquid chromatography-ion trap mass spectrometry. Electrophoresis, 2000, 21:3058-68 [pdf]
  • Che FY, Xia QC.Thiophosphorylation and Fluorescent Labeling of Substrate Peptide of Protein Kinase. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000, 32:69-73
  • Yang YH, Chen H, Yu C, Zeng R, Cui DF, Wu XF, Li BL.In Vitro Amidating Processing of Products Expressed by Gene Engineering. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000, 32:312-5.
  • Li MY, Liu ZH, Shao XX, Xia QC, Zhou GM, Lin QS, Cui DF. Synthesis of Human Proinsulin C-peptide and Preparation of Specific Antibody to It. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000, 32:665-8.
  • Li-Rong Yu, Nan Wang, Ggo-De Wu, Yong-Hua Xu and Qi-Chang Xia. Two-dimensional gel electrophoresis analysis of the proteome expressed in the human hepatoma cell line BEL-7404 and normal liver cell line L-02. Chinese Science Bulletin, 2000, 45:1113-21
  • Rong Zeng, Li-Rong Yu, Xiao-Xia Shao and Qi-Chang Xia. Identification of two-dimensional lectrophoresis-separated proteins in human hepatoma cell by electrospray ion trap mass spectrometry. Chinese Science Bulletin, 2000, 45:1509-14
  • Qiang Xu, Rong Zeng, Xiang-Fu Wu, Ke-Yi Wang. Expression of isolated C-type carbohydrate recognition domains. Biochemical and Biophysical Research Communications, 2000, 271:677-81
  • FY Che, XX Shao and QC Xia. Identification of phosphorylation sites of proteins by high performance liquid chromatography-electrospray ionization-quadrupole ion trap mass spectrometry. Science in China (Series C), 2000, 43:561-8
  • 1999

  • Rong Zeng, Qiang Xu, Xiao-Xia Shao, Ke-Yi Wang, Qi-Chang Xia. Characterization and analysis of a noval glycoprotein from snake Venom using liquid chromatography-electrospray mass spectrometry and Edman degradation. Eur. J. Biochem, 1999, 266:352-8
  • Fa-Yun Che, Jin-Fang Song, Rong Zeng, Ke-Yi Wang and Qi-Chang Xia. Analysis of 8-aminonapthalene-1,3,6- trisulfonate-derivatized oligosaccharides by capillary electrophoresis-electrospray ionization quadrupole ion trap mass spectrometry. J. Chromatography A, 1999, 858:229-38
  • Fa-Yun Che, Ke-Yi Wang, Qi-Chang Xia. Determination of sialic acid content in glycoproteins by capillary zone electrophoresis. Electrophoresis, 1999, 20:2930-7
  • Tao Liu, Xiao-Xia Shao, Rong Zeng, Qi-Chang Xia. Analysis of recombinant and modified proteins by capillary zone electrophoresis/electrospray ionization-tandem mass spectrometry. J. Chromatography A, 1999, 855:695-707
  • Qiang Xu, Xiang-Fu Wu, Qi-Chang Xia, Ke-Yi Wang. Cloning of a galactose-binding lectin from the venom of stejnegeri Schmidt. Biochem. J, 1999, 314:733-7
  • Xiong YM, Lan ZD, Wang M, Liu B, Liu XQ, Fei H, Xu LG, Xia QC, Wang CG, Wang DC, Chi CW. Molecular characterization of a new excitatory insect neurotoxin with an analgesic effect on mice from the scorpion Buthus martensi Karsch. Toxicon. 1999, 37:1165-80.
  • Fa-Yun Che, Jin-Fang Song, Xiao-Xia Shao, Ke-Yi Wang and Qi-Chang Xia. Comparative study on the distribution of ovalbumin glycoforms by capillary electrophoresis. J. Chromatography A, 1999, 849:599-608
  • Zeng R, Shao XX, Xia QC. Peptide Mapping and Primary Structure Analysis of hEGFby Mass Spectrometry. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999, 31:31-6.
  • Liu T, Zeng R, Shao XX, Xia QC. Protein and Peptide Identification by Capillary Zone Electrophoresis-Tandem Mass Spectrometry. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999, 31:425-32.
  • Liu T, Shen X, Wu GD, Xia QC, Wang SN, Wu ZJ, Hu W. Application of Free-flow Electrophoresis to the Separation of Murine Lymphocytes. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999, 31:590-3.
  • Zeng R, Shao XX, Wang KY, Xia QC. Analysis of Peptide Mapping Glycosylated Site and Glycan Structure in Ribonuclease B by Liquid Chromatography-Electrospray Mass Spectrometry. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999, 31:695-700.
  • 1998

  • Song JF, Liu T, Shen X, Wu GD, Xia QC. Application of free-flow electrophoresis to the purification of trichosanthin from a crude product of acetone fractional precipitation. Electrophoresis. 1998, 19:1097-103.
  • Che FY, Liu ZY, Wang KY, Xia QC. Analysis of Monosaccharides Derivatized with 2-aminoacridone by Capillary Electrophoresis. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998, 30:251-6.
  • Che FY, Liu ZY, Wang KY, Xia QC. Separation and Electrophoretic Behaviour of 8-aminonaphthalene-1,3,6-trisulfonic Acid-derivatized Oligosaccharides by Capillary Electrophoresis. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998, 30:495-500.
  • Zeng R, Xu LG, Xia QC. C-terminal Analysis of Proteins and Peptides. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998, 30:459-64.

  • RESEARCH CENTER FOR PROTEOME ANALYSIS  All RIGHTS RESEARVED
    320 Yueyang Road, ShanghaiP.R.China  200031
    TEL: 54920169 54920167  FAX: 54920171  EMAIL:proteomics@sibs.ac.cn